TCONS_00066683-protein (polypeptide) - C. hemisphaerica

Overview


Name	TCONS_00066683-protein
Unique Name	TCONS_00066683-protein
Type	polypeptide
Organism	Clytia hemisphaerica (Jellyfish)
Sequence length	814

Sequence

The following sequences are available for this feature:

polypeptide sequence

polypeptide sequence

>TCONS_00066683-protein ID=TCONS_00066683-protein|Name=TCONS_00066683-protein|organism=Clytia hemisphaerica|type=polypeptide|length=814bp
MAAAGIWDRLHPDEFTKLQEYTKYSKRRLKDVLQEFREGYHEGYDPEQPM
GYDGFKLFMSVYLGQSVSEELMQTLFWTFHKKVPRGDAKSPDLPNTILRK
ILVENGNVSQESLDQLNESSLSRIAKGARRATVVATATVVAGDIPRRAIS
EAELSRIDKTNDEMLTPITKRRLFLDTMTHLPMIEIKEIACYLSLLEGGD
VKDKLEFVFRVYDSDNNDYLDGEELESIVSQMLRVAEYLGWDTTALKPIL
TDMLADMDYDSDGQISLEEWVKGGMNNIPFLVLMGMEIKVDEEGKHQWAM
KHFKTQAFCNICHGVLAGFGRKQGLQCIFCRFTCHERCVQRSPNSCISTY
TQNKTKRKVTEMDHHWVEGNCPGKCTKCNKTIKNNCLTGLHCVWCSAQVH
NRCVTNMPVMCSLGPHKVHILPPICITPASKEKAEKVVKDKKNSVISYDG
IPMQITPLPDTKPLIAFINPKSGGRQGARLLNKFRYLLNPRQVFNLAEGG
PFPGLKFFAQVPDFRVLCCGGDGTAGWILATIDRLTSLKQRPPMAILPLG
TGNDLARCLGWGGGYEGGSLDKVLQKVLKSSVVMMDRWQIDCDNFEKTSI
EGDIMPQNIMNNYFSIGVDAAIALKFHLQREKNPEKFNSRFKNKLRYFQA
GTSEQLAATCTKLSNFLEIICDGKKLELPSLEGIAILNIPSIYGGANIWG
EPEKKRKRRKKSSGSSGDEPESLGSVSQDISDRKLEVVGLENSLYVGQII
AGVRQHGLRLAQCSTVVIKIMKTMPMQIDGEPWMQAPAQITINHKNQSPM
CVAPRNEKRGWFRF

Run BLAST on NCBI

Gene-mRNA-Prot

This polypeptide comes from the following gene feature:


Feature Name	Unique Name	Species	Type
XLOC_041707	XLOC_041707	Clytia hemisphaerica	gene

This polypeptide derives from the following transcript feature(s):


Feature Name	Unique Name	Species	Type
TCONS_00066683	TCONS_00066683	Clytia hemisphaerica	transcript

Annotated Terms

The following terms have been associated with this polypeptide:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR001206	Diacylglycerol_kinase_cat_dom
IPR000756	Diacylglycerol_kin_accessory
IPR002219	PE/DAG-bd
IPR017438	ATP-NAD_kinase_dom_1
IPR029477	DAG_kinase_typeI_N
IPR018247	EF_Hand_1_Ca_BS
IPR016064	NAD/diacylglycerol_kinase
IPR011992	EF-hand-dom_pair

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding
GO:0016301	kinase activity
GO:0004143	diacylglycerol kinase activity
GO:0003951	NAD+ kinase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0007205	protein kinase C-activating G-protein coupled receptor signaling pathway
GO:0035556	intracellular signal transduction
GO:0008152	metabolic process

GO Annotation

GO Assignments

This polypeptide is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0035556	intracellular signal transduction
biological_process	GO:0008152	metabolic process
biological_process	GO:0007205	protein kinase C-activating G-protein coupled receptor signaling pathway
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004143	diacylglycerol kinase activity
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0003951	NAD+ kinase activity

InterPro

Analysis Name: InterPro Annotations of C. hemisphaerica v1.0
Date Performed: 2017-06-01

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 249..277 e-value: 1.0 score: 17.7 coord: 204..232 e-value: 0.16 score: 21.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 203..269 e-value: 6.2E-8 score: 32.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 245..280 score: 7.707
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 200..235 score: 11.333
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 204..273 e-value: 5.80237E-12 score: 61.4097
IPR001206	Diacylglycerol kinase, catalytic domain	SMART	SM00046	dagk_c4a_7	coord: 463..588 e-value: 5.3E-56 score: 202.0
IPR001206	Diacylglycerol kinase, catalytic domain	PFAM	PF00781	DAGK_cat	coord: 464..576 e-value: 2.5E-25 score: 88.4
IPR001206	Diacylglycerol kinase, catalytic domain	PROSITE	PS50146	DAGK	coord: 459..594 score: 44.314
IPR000756	Diacylglycerol kinase, accessory domain	SMART	SM00045	dagk_c4b_2	coord: 609..782 e-value: 1.6E-81 score: 286.8
IPR000756	Diacylglycerol kinase, accessory domain	PFAM	PF00609	DAGK_acc	coord: 609..782 e-value: 9.1E-52 score: 175.5
IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	SMART	SM00109	c1_12	coord: 296..346 e-value: 1.7E-7 score: 40.9 coord: 364..411 e-value: 1.7E-5 score: 34.3
IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	PFAM	PF00130	C1_1	coord: 296..347 e-value: 2.7E-10 score: 39.9 coord: 364..412 e-value: 2.6E-9 score: 36.7
IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	PROSITE	PS00479	ZF_DAG_PE_1	coord: 296..346
IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	PROSITE	PS00479	ZF_DAG_PE_1	coord: 364..411
IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	PROSITE	PS50081	ZF_DAG_PE_2	coord: 363..411 score: 11.744
IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	PROSITE	PS50081	ZF_DAG_PE_2	coord: 295..346 score: 13.597
IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	CDD	cd00029	C1	coord: 364..411 e-value: 1.24942E-8 score: 51.3388
IPR002219	Protein kinase C-like, phorbol ester/diacylglycerol-binding domain	CDD	cd00029	C1	coord: 296..346 e-value: 2.35788E-12 score: 62.1244
IPR017438	Inorganic polyphosphate/ATP-NAD kinase, domain 1	GENE3D	3.40.50.10330		coord: 458..602 e-value: 3.9E-33 score: 113.5
None	No IPR available	GENE3D	3.30.60.20		coord: 287..360 e-value: 3.0E-15 score: 55.3
None	No IPR available	GENE3D	1.10.238.10		coord: 205..283 e-value: 4.7E-12 score: 45.1
None	No IPR available	GENE3D	1.10.238.110		coord: 3..204 e-value: 3.2E-29 score: 101.2
None	No IPR available	GENE3D	3.30.60.20		coord: 361..412 e-value: 8.6E-10 score: 38.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 703..727
None	No IPR available	SUPERFAMILY	57889	Cysteine-rich domain	coord: 354..416
None	No IPR available	SUPERFAMILY	57889	Cysteine-rich domain	coord: 279..348
IPR029477	Diacylglycerol kinase type I, N-terminal	PFAM	PF14513	DAG_kinase_N	coord: 7..199 e-value: 3.7E-31 score: 108.5
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 258..270
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 213..225
IPR016064	NAD kinase/diacylglycerol kinase-like domain	SUPERFAMILY	111331	NAD kinase/diacylglycerol kinase-like	coord: 728..795 coord: 459..701
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 183..292
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 7..91 coord: 181..207

Blast

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BLAST of TCONS_00066683-protein vs. Swiss-Prot (Human)
Match: DGKB (Diacylglycerol kinase beta OS=Homo sapiens GN=DGKB PE=2 SV=2)

HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0
Identity = 400/842 (47.51%), Postives = 525/842 (62.35%), Query Frame = 0

Query:    7 WDRLHPDEFTKLQEYTKYSKRRLKDVLQEFR-EGYHEGYDPE-------QPMGYDGFKLFMSVYLGQSVSEELMQTLFWTFHKKVPRGDAKSPDLPNTILRKILVENGNVSQESLDQLNESSLSRIAKGARRATVVATATVVAGDIPRRAISEAELSRIDKTNDEMLTPITKRRLFLDTMTHLPMIEIKEIACYLSLLEGGDVKDKLEFVFRVYDSDNNDYLDGEELESIVSQMLRVAEYLGWDTTALKPILTDMLADMDYDSDGQISLEEWVKGGMNNIPFLVLMGMEIKVDEEGKHQWAMKHFKTQAFCNICHGVLAGFGRKQGLQCIFCRFTCHERCVQRSPNSCISTYTQNKTKRKVTEMDHHWVEGNCPGKCTKCNKTIK-NNCLTGLHCVWCSAQVHNRCVTNMPVMCSLGPHKVHILPPICITPA-----------------SKEKAEKVVKDKKNSVI-----------SYDGIPMQITPLPDTKPLIAFINPKSGGRQGARLLNKFRYLLNPRQVFNLAEGGPFPGLKFFAQVPDFRVLCCGGDGTAGWILATIDRLTSLKQRPPMAILPLGTGNDLARCLGWGGGYEGGSLDKVLQKVLKSSVVMMDRWQIDCDNFEKTSIEGDIMPQNIMNNYFSIGVDAAIALKFHLQREKNPEKFNSRFKNKLRYFQAGTSEQLAATCTKLSNFLEIICDGKKLEL--PSLEGIAILNIPSIYGGANIWGEPEKKRKRR---KKSSG--SSGDEPESLGSVSQDISDRKLEVVGLENSLYVGQIIAGVRQHGLRLAQCSTVVIKIMKTMPMQIDGEPWMQAPAQITINHKNQSPMCVAP 804
            W  L P EF++LQ+Y +YS ++LKDVL+EF   G    Y+PE       Q + ++GFKLFM  +L   + ++    LF +F  K P         P    +  L+  G                R+ KGA                P R  S A     +                        +I +K+I CYLSLLE G  +DKLEF+FR+YD+D N +LD  ELE+I+SQM+ VAEYL WD T L PIL +M+ ++DYD DG +SLEEW++GGM  IP LVL+G+E  V ++G+H W +KHF   A+CN+C  +L G G KQGL C FC++T HERCV R+P SCI TY   K+KR    M H+WVEGNCP KC KC+KT+K    LTGLHCVWC   +HN+C +++   C  GP K HILPP  I P                  S  K EK    + N VI           + DG  +Q+TP+P T PL+ F+NPKSGG+QG R+  KF+YLLNPRQV++L+  GP PGL FF  VPDFRVL CGGDGT GW+L  I++  ++ + PP+AILPLGTGNDLARCL WGGGYEG +L K+L+ +  S+ +M+DRW+ +    +K   +GD +P +I+NNYFSIGVDA+IA +FH+ REK+PEKFNSR KNK  YF+ GTSE  +ATC KL   +EI CDG +++L   SLEGIAILNIPS++GG+N+WGE +K+R  R   KK S   ++  + + L   SQD+SD+ LEVVGLE ++ +GQI  G++  G RLAQCS VVI+  K++PMQIDGEPWMQ P  I I HKNQ+PM + P
Sbjct:    7 WAHLSPSEFSQLQKYAEYSTKKLKDVLEEFHGNGVLAKYNPEGKQDILNQTIDFEGFKLFMKTFLEAELPDDFTAHLFMSFSNKFPHSS------PMVKSKPALLSGG---------------LRMNKGAI--------------TPPRTTSPANTCSPE------------------------VIHLKDIVCYLSLLERGRPEDKLEFMFRLYDTDGNGFLDSSELENIISQMMHVAEYLEWDVTELNPILHEMMEEIDYDHDGTVSLEEWIQGGMTTIPLLVLLGLENNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGVG-KQGLCCSFCKYTVHERCVARAPPSCIKTYV--KSKRNTDVMHHYWVEGNCPTKCDKCHKTVKCYQGLTGLHCVWCQITLHNKCASHLKPECDCGPLKDHILPPTTICPVVLQTLPTSGVSVPEERQSTVKKEKSGSQQPNKVIDKNKMQRANSVTVDGQGLQVTPVPGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSLSGNGPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEK-ANVGKHPPVAILPLGTGNDLARCLRWGGGYEGENLMKILKDIENSTEIMLDRWKFEVIPNDKDE-KGDPVPYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKRRSHRRIEKKGSDKRTTVTDAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQIDGEPWMQTPCTIKITHKNQAPMLMGP 784

The following BLAST results are available for this feature:

BLAST of TCONS_00066683-protein vs. Swiss-Prot (Human)

BLAST of TCONS_00066683-protein vs. Swiss-Prot (Human)
Analysis Date: 2018-01-31 (Blastp Clytia hemisphaerica v1.0 proteins vs SwissProt (Homo sapiens))
Total hits: 1

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Match Name	E-value	Identity	Description
DGKB	0.000e+0	47.51	Diacylglycerol kinase beta OS=Homo sapiens GN=DGKB...	[more]

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TCONS_00066683-protein (polypeptide) - C. hemisphaerica

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