TCONS_00058467-protein (polypeptide) - C. hemisphaerica

Overview
NameTCONS_00058467-protein
Unique NameTCONS_00058467-protein
Typepolypeptide
OrganismClytia hemisphaerica (Jellyfish)
Sequence length969

Sequence
The following sequences are available for this feature:

polypeptide sequence

>TCONS_00058467-protein ID=TCONS_00058467-protein|Name=TCONS_00058467-protein|organism=Clytia hemisphaerica|type=polypeptide|length=969bp
MIELNVDKKNQPAVVLLTVEKLKSLNEKVQVNQADKTFVKISSDTTIEGE
NAVSRYLARQFPNAQLYGSTALEMSQIDSWLDFSTLYLKNSSEIKDAIVE
LNKSLKMSTYLVGQGFTLADFAIWAALKDNSKWSASDDEVSRWFKFASEN
GIFKAVSAKTTKSSSSNQGGSKKGDGKPLEEGGKFIDLPGAEMGKVVTRF
PPEASGYLHIGHAKAALLNQAYKEIYDGKLIMRFDDTNPAKENAHFEQII
LEDIKLLKLTPDMYSRTSDHFDKIEVFAEQLIRQGDAYCDCTPSEEMRKC
REERQASIYRDQTPEEAMRIFQEMKKGSPEGLANCLRGKVDYASDNGCMR
DPVLYRCRNEEHISTGDKYKAYPTYDFACPIVDSVEGVTHTMRTSEYDDR
DVQYFWMLEKLGLRKPMIYSYSRLNLINTVLSKRKLTYLVDEGLVDGWDD
PRFPTVRGVLRRGLTIPGLIEFIKAQGASKANVVMGWDKIWAFNKKMIDP
VAPRYNALLKNDVVPVNVNNANESSTKYPRHPKNEEVGEKNVYYSSKVFM
EGEDAAAIKEGDLVTFINWGNLRIKGINKEGDKVVSVDAVTELDNKDFKK
TLKVTWLAEHSSAPFVPTKCFFFDNIITKDVLGKDDNFKDFIGKDTKREN
EMLGDPELKNLKKGDIIQIQRRGYFICDQPYEPPSRHSGRESPCMLFHIP
DGSTAGMMNQKNDKKNASGGGGGGSNSSATARSNKKKQQKQTSPQKESKA
NLSTSATTAASDASGHTPEQQKLYDSVTAQGDKIRSLKAEKAAKDAILAE
VTVLKDLKAQYKAATGREYVAAPTANTSKPVEKKMAPVASTTVSLSPEAE
SVVQKITEQGNKIRDMKTNKAAKEALQPEIDQLLKLKSEYKAITGSDYQA
PGAGSSKKDKKNKENKQPKQPKXVRKTRRAKRTNSQSNPRKRKRRQRKRR
RPPPPRPMMDRNRNKHVSV
Run BLAST on NCBI
Gene-mRNA-Prot
This polypeptide comes from the following gene feature:
Feature NameUnique NameSpeciesType
XLOC_036841XLOC_036841Clytia hemisphaericagene
This polypeptide derives from the following transcript feature(s):
Feature NameUnique NameSpeciesType
TCONS_00058467TCONS_00058467Clytia hemisphaericatranscript
Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR000924Glu/Gln-tRNA-synth
IPR000738WHEP-TRS_dom
IPR020056Rbsml_L25/Gln-tRNA_synth_b-brl
IPR020059Glu/Gln-tRNA-synth_Ib_codon-bd
IPR014729Rossmann-like_a/b/a_fold
IPR020061Glu/Gln-tRNA-synth_Ib_a-bdl
IPR020058Glu/Gln-tRNA-synth_Ib_cat-dom
IPR004526Glu-tRNA-synth_arc/euk
IPR001412aa-tRNA-synth_I_CS
IPR011035Ribosomal_L25/Gln-tRNA_synth
IPR009068S15_NS1_RNA-bd
IPR010987Glutathione-S-Trfase_C-like
Vocabulary: Biological Process
TermDefinition
GO:0043039tRNA aminoacylation
GO:0006418tRNA aminoacylation for protein translation
GO:0006424glutamyl-tRNA aminoacylation
GO:0006412translation
Vocabulary: Molecular Function
TermDefinition
GO:0000166nucleotide binding
GO:0005524ATP binding
GO:0016876ligase activity, forming aminoacyl-tRNA and related compounds
GO:0004812aminoacyl-tRNA ligase activity
GO:0004818glutamate-tRNA ligase activity
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
GO Annotation
GO Assignments
This polypeptide is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006424 glutamyl-tRNA aminoacylation
biological_process GO:0006412 translation
biological_process GO:0043039 tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004818 glutamate-tRNA ligase activity
molecular_function GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds
molecular_function GO:0000166 nucleotide binding
InterPro
Analysis Name: InterPro Annotations of C. hemisphaerica v1.0
Date Performed: 2017-06-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000924Glutamyl/glutaminyl-tRNA synthetasePRINTSPR00987TRNASYNTHGLUcoord: 389..397
score: 40.28
coord: 373..383
score: 55.0
coord: 199..211
score: 60.0
coord: 213..224
score: 38.12
coord: 228..241
score: 47.68
IPR000738WHEP-TRS domainSMARTSM00991WHEP_TRS_a_2_a_3_acoord: 773..829
e-value: 1.1E-20
score: 84.7
coord: 852..911
e-value: 9.1E-16
score: 68.4
IPR000738WHEP-TRS domainPFAMPF00458WHEP-TRScoord: 854..901
e-value: 5.0E-17
score: 61.7
coord: 773..823
e-value: 4.1E-18
score: 65.2
IPR000738WHEP-TRS domainPROSITEPS51185WHEP_TRS_2coord: 769..825
score: 13.219
IPR000738WHEP-TRS domainPROSITEPS51185WHEP_TRS_2coord: 848..904
score: 12.182
IPR020056Ribosomal protein L25/Gln-tRNA synthetase, beta-barrel domainGENE3D2.40.240.10coord: 511..610
e-value: 1.3E-8
score: 34.3
IPR020056Ribosomal protein L25/Gln-tRNA synthetase, beta-barrel domainGENE3D2.40.240.10coord: 611..714
e-value: 7.5E-15
score: 54.5
IPR020059Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domainPFAMPF03950tRNA-synt_1c_Ccoord: 502..678
e-value: 2.7E-33
score: 115.2
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620coord: 309..425
e-value: 5.7E-8
score: 31.9
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620coord: 196..308
e-value: 1.4E-30
score: 105.2
IPR020061Glutamyl/glutaminyl-tRNA synthetase, class Ib, alpha-bundle domainGENE3D1.10.1160.10coord: 426..500
e-value: 9.9E-28
score: 95.4
NoneNo IPR availableGENE3D3.40.30.10coord: 8..73
e-value: 9.0E-5
score: 22.4
NoneNo IPR availableGENE3D1.10.287.10coord: 846..899
e-value: 5.8E-14
score: 51.3
coord: 766..820
e-value: 1.6E-18
score: 65.9
NoneNo IPR availableGENE3D1.20.1050.10coord: 74..160
e-value: 1.6E-7
score: 31.0
NoneNo IPR availableCDDcd00936WEPRS_RNAcoord: 852..901
e-value: 8.07449E-13
score: 63.7936
NoneNo IPR availableCDDcd00936WEPRS_RNAcoord: 773..822
e-value: 1.14317E-14
score: 69.1864
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 195..506
IPR020058Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domainPFAMPF00749tRNA-synt_1ccoord: 195..499
e-value: 1.1E-102
score: 343.0
IPR004526Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolicTIGRFAMTIGR00463TIGR00463coord: 177..679
e-value: 5.4E-134
score: 445.7
IPR001412Aminoacyl-tRNA synthetase, class I, conserved sitePROSITEPS00178AA_TRNA_LIGASE_Icoord: 202..213
IPR011035Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domainSUPERFAMILY50715Ribosomal protein L25-likecoord: 502..702
IPR009068S15/NS1, RNA-bindingSUPERFAMILY47060S15/NS1 RNA-binding domaincoord: 773..822
IPR009068S15/NS1, RNA-bindingSUPERFAMILY47060S15/NS1 RNA-binding domaincoord: 850..901
IPR010987Glutathione S-transferase, C-terminal-likeSUPERFAMILY47616GST C-terminal domain-likecoord: 51..147

Blast
BLAST of TCONS_00058467-protein vs. Swiss-Prot (Human)
Match: SYEP (Bifunctional glutamate/proline--tRNA ligase OS=Homo sapiens GN=EPRS PE=1 SV=5)

HSP 1 Score: 872.848 bits (2254), Expect = 0.000e+0
Identity = 446/927 (48.11%), Postives = 601/927 (64.83%), Query Frame = 0
Query:    2 IELNVDKKNQPAVVLLTVEKLKSLNEKVQVNQADKTFVKISSDTTIEGENAVSRYLARQFPNAQLYGSTALEMSQIDSWLDFSTLYLKNSSEIKDAIVELNKSLKMSTYLVGQGFTLADFAIWAALKDNSKWSA------SDDEVSRWFKFASENGIFKAVSAKTTKSSSSNQGGSKKGDGKPLEEGGKFIDLPGAEMGKVVTRFPPEASGYLHIGHAKAALLNQAYKEIYDGKLIMRFDDTNPAKENAHFEQIILEDIKLLKLTPDMYSRTSDHFDKIEVFAEQLIRQGDAYCDCTPSEEMRKCREERQASIYRDQTPEEAMRIFQEMKKGSPEGLANCLRGKVDYASDNGCMRDPVLYRCRNEEHISTGDKYKAYPTYDFACPIVDSVEGVTHTMRTSEYDDRDVQYFWMLEKLGLRKPMIYSYSRLNLINTVLSKRKLTYLVDEGLVDGWDDPRFPTVRGVLRRGLTIPGLIEFIKAQGASKANVVMGWDKIWAFNKKMIDPVAPRYNALLKNDVVPVNVNNANESSTKYPRHPKNEEVGEKNVYYSSKVFMEGEDAAAIKEGDLVTFINWGNLRIKGINKEGD-KVVSVDAVTELDNKDFKKTLKVTWLAEHSSAPFVPTKCFFFDNIITKDVLGKDDNFKDFIGKDTKRENEMLGDPELKNLKKGDIIQIQRRGYFICDQPYEPPSRHSGRESPCMLFHIPDGSTAGMMNQKNDKKNASGGGGGGSNSSATARSNKKKQQKQTSPQKESKANLSTSATTAASDASGHTPEQQKLYDSVTAQGDKIRSLKAEKAAKDAILAEVTVLKDLKAQYKAATGREYVAAPTANTSKPVEKKMAPVASTTVSLSPEAESVVQKITEQGNKIRDMKTNKAAKEALQPEIDQLLKLKSEYKAITGSDY---QAPGAGSSKKDKKNKENKQP 918
            + L V+  + P   LL VE +K  +  + V +  +  + +S +      N++ RYLAR    A LYGS  +E ++ID WL+FS   L +       I ELN  L + TYLVG   +LAD  +WA LK N+ W        +   V RWF F      F++V  K   S++  +   +K      ++ GKF++LPGAEMGKV  RFPPEASGYLHIGHAKAALLNQ Y+  + GKLIMRFDDTNP KE   FE++ILED+ +L + PD ++ TSDHF+ I  +AE+LI++G AY D TP+E+M+  RE+R  S +R    E+ +++++EMKKGS  G + CLR K+D +S+NGCMRDP LYRC+ + H  TG+KY  YPTYDFACPIVDS+EGVTH +RT+EY DRD Q++W++E LG+RKP I+ YSRLNL NTVLSKRKLT+ V+EGLVDGWDDPRFPTVRGVLRRG+T+ GL +FI AQG+S++ V M WDKIWAFNKK+IDPVAPRY ALLK +V+PVNV  A E   +  +HPKN EVG K V+YS KVF+EG DA    EG++VTFINWGNL I  I+K  D K++S+DA   L+NKD+KKT KVTWLAE + A  +P  C  ++++ITK VLGKD++FK ++ K++K E  MLGDP LK+LKKGDIIQ+QRRG+FICDQPYEP S +S +E+PC+L +IPDG T  M                         S  K++ K  + + E+ A      T + ++    + +   LY+ V  QGD +R LKA+KA K+ + A V  L  LKA+YK  TG+EY          P  +    ++S + +   E++S+  ++  QG  +R +K  K+ K  +   ++ LL LK++YK  TG +Y   Q P + SS  D     N +P
Sbjct:    4 LSLTVNSGDPPLGALLAVEHVKD-DVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKK-----QDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEM-----------------------PTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEY------KPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSS--DSSPTRNSEP 893          

HSP 2 Score: 87.8113 bits (216), Expect = 1.639e-17
Identity = 52/137 (37.96%), Postives = 79/137 (57.66%), Query Frame = 0
Query:  769 EQQKLYDSVTAQGDKIRSLKAEKAAKDAILAEVTVLKDLKAQYKAATGREYVAA--PTANTSKPVEKKMAPVASTTVSLSPEAESVVQKITEQGNKIRDMKTNKAAKEALQPEIDQLLKLKSEYKAITGSDYQAPGA 903
            E + LYD V AQG+ +R LKAEK+ K  I   V  L  LKAQYK  TG+EY+    P + +S     + +  A      +PEA+ +  K+  QG  +R +KT KA K+ +   + +LL+LK++YK++ G +Y+   A
Sbjct:  822 ESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLE---TPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSA 955          
The following BLAST results are available for this feature:
BLAST of TCONS_00058467-protein vs. Swiss-Prot (Human)
Analysis Date: 2018-01-31 (Blastp Clytia hemisphaerica v1.0 proteins vs SwissProt (Homo sapiens))
Total hits: 1
Match NameE-valueIdentityDescription
SYEP1.639e-1748.11Bifunctional glutamate/proline--tRNA ligase OS=Hom... [more]
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